With the recent approval of anti- -amyloid treatment for Alzheimer's disease, a demand has emerged for scalable, convenient and accurate estimations of brain A burden for the detection of Alzheimer's disease that would enable timely, accurate and reliable diagnosis in one's primary care physician's office as called for recently by World Health Organization.
This study investigated the protective and regenerative effects of a water extract of Phalaenopsis orchid flower, an ornamental plant endemic to Taiwan, on high glucose-induced cellular senescence in human dermal fibroblasts, with a focus on the Nrf2/HO-1 antioxidant pathway. Cytotoxicity, cellular senescence, and ROS production were respectively assessed using MTT assay, senescence-associated -galactosidase staining, and DCFDA-cellular reactive oxygen species assay. Fibroblasts treated with WEPF under HG conditions exhibited reduced senescence-associated -galactosidase activity, lower ROS levels, and attenuated cell cycle arrest. Protein expression profiling revealed suppression of the p53/p21Waf1, and p16INK4a/Rb pathways and decreased matrix metalloproteinase-1 expression. Mechanistically, WEPF exerted its effects by activating the Nrf2/HO-1 axis and restoring the expression of senescence marker protein-30, thereby promoting fibroblast repair and reducing pro-inflammatory signaling. These findings support the potential of WEPF as a botanical therapeutic agent for diabetic wound healing and age-related skin deterioration.